TRIS 醋酸盐

应用

Tris-乙酸缓冲液EDTA缓冲液作为运行缓冲液，用于人类乳头状瘤病毒DNA的琼脂糖凝胶电泳以及转录的RNA样品。TAE 电泳缓冲液是 DNA 琼脂糖凝胶电泳最常用的缓冲液，还用于非变性 RNA 琼脂糖凝胶电泳。双链 DNA 在 TAE 中往往比在其他缓冲液中移动得更快，但在长时电泳期间也会因缓冲液离子耗尽而无法泳动。在长时电泳期间进行缓冲液循环或缓冲液更换可弥补较低的缓冲能力。稀释浓缩的 TAE 缓冲液得到 1× TAE 缓冲液，含有 40mM Tris 乙酸盐和 1mM EDTA，pH 为 8.3。1× TAE 缓冲液既可用于琼脂糖凝胶中，也可用作电泳缓冲液。为达到最大分辨率，建议外加电压低于 5V/cm（单位电极间的距离）。

适用性

原理

制备说明

用 18 兆欧水制备的溶液采用经生物技术性能认证的 Trizma 碱 (T6066) 和分子生物学试剂 EDTA (E5134) 制备。溶液还含有乙酸 (A6283)；粉末状混合物含有 Trizma 醋酸盐 (T1258)。

外形

Description

Tris Acetate-EDTA buffer has been used as run buffer for the agarose gel electrophoresis of human papillomavirus DNA and transcribed RNA samples.TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.